NATIONAL INSTITUTE OF CHOLERA AND ENTERIC DISEASES (NICED) ANNUAL REPORT 2004-2005

Particulars	Page No.
Preface	1
1. Study On Hospital Surveillance	14
1.1. Hospital based surveillance system for diarrhoeal diseases	14
2. Community Based Studies	15
2.1. Acute lower respiratory tract infection (ALRI) and diarrhoea in rural children below two years in relation to feeding practices with particular reference to breast feeding : A community based study	15
2.2 . An operational study on Effect of zinc supplementation on reduction of diarrhoeal morbidity amongst rural children. (In collaboration with Health Department, Government of West Bengal)	15
2.3. Epidemiology of typhoid fever in a rural and urban slum community of West Bengal	19
2.4 Identifying environmental risk factors for endemic diarrhoeal diseases in West Bengal, India: a remote sens-ing- geographic information system(GIS) approach	21
3. Clinical Studies	22
3.1. Evaluation of comparative efficacy of Cefuroxime axetil and Tetracycline in the treatment of cholera in adults	22
3.2. Impact of Lactobacillus (Lactic acid bacteria) in children with acute watery diarrhoea	22
4. Studies on Vibrio cholerae	25
4.1. Studies on the structure-function relationship of Vibrio cholerae hemolysin (HlyA)	25
4.2. Sequential studies of Vibrio cholerae 0139 and some of its defined virulence phenotypes on intestinal pathology in rabbit ileal loop model	26
4.3 Vibriocidal assay on rough vibro cholerae strains with acute and convalescent sera collected from cholera patients and implication of gene(s) for the rough phenotype	26
4.4. Spread of cholera with vibro clolerae 01,Serotype Inaba in India.	27
5. Studies on Vibrio cholerae Phages	30
5.1. Further application of phage typing scheme for discriminating strains of Vibrio cholerae O139	30
5.2. Nationwide screening of phage types of V. cholerae O1 biotype EltTor	30
5.3. Molecular analysis of Vibrio cholerae bacteriophages: cloning and sequencing of phage DNA	30

6. Studies on Shigella species	36
6.1. Molecular characterization of multi-drug resistant Shigella flexneri in Kolkata	36
6.2. Up-regulation of mucosal immune response by porin of Shigella dysenteriae type 1: Study of Toll-like receptor, costimulatory molecule and immunoglobulin expression on murine B-1 cell	37
6.3. Antigenic recognition of Shigella dysenteriae outer membrane proteins using human convalescent sera and to evaluate their role in cell-mediated immune response in shigellosis	38
7. Studies on Escherichia coli	39
7.1.Studies on the binding of Escherichia coli.....	39
7.2. Virulence Characteristics and molecular Epidemiology........	40
7.3. Electron microscopic study of ultra structures associated with Intestinal cell adherence of enteroaggregative Escherichia coliisolated from acute hospitalized diarrhea cases and healthycontrol children from Kolkata 63	43
7.4. Developed of a PCR based assay for detection of .......	45
8. Studies on Helicobacter pylori	47
8.1. Correlation of histology with genotypes of Helicobacterpylori isolated.......	48

8.2. Most Helicobacter pylori starins of Kolkata are resistant to ......	48
9. Studies on other bacterial pathogen	51
9.1. Mode of action of Yersinia enterocolitica heat stable enterotoxin (YSTa) in rat intestinal epithelial cell	51
10. Studies on Diarrhoeagenic Parasites	52
10.1. Studies on rRNA processing in Giardia lamblia	52
10.2. Studies on electron dense granules of Entamoeba histolytica containing collagenase activity	54
11. Studies on Viral Pathogens	61
11.1. Genotyping of Group a rotavirus strains:	61
11.2. Studies on astrovirus causing acute watery diarrhoea among chidren in kolkata ,India	62
11.3. Studies on human caliciviruses by Reverse Transcripti on-Polymerase Chain Reaction [RT-PCR] to detect the presence of Norwalk like virus [NLV] and Sapporo like virus [SLV]	63
12. Studies on HIV/AIDS	68
12.1. Study on sexually transmitted infections among female sex workers of West Bengal	68
13. Epidemic Investigations	74
13.1. Investigation of acute diarrhoeal dieses at Tripura	74
13.2. Investigation diarrhoeal dieses out break in delhi Tripura	76
13.3. Investigation of out break ...	76
13.4. Testing of stool and drinking water samples received from other parts of West Bengal	76
13.5. Epidemic Investigation of diarrhoeal out break in Garulia, North 24 Parganas	77
13.6. diarrhoeal dieses out break in different words of Behala ,Kolkata ,West Bengal	80
14. Activities of National HIV Reference Centre of NICED, Kolkata	94
14.1 Phylogenetic analysis of HIV-1	95
15. NICED-JICA Project	100
15.1 Summary of Activity of the project in FY 2004-05 and Activity of the project	100
16. IVI-NICED Collaborative Project	108
16.1. Surveillance for typhoid fever and cholera in eastern Kolkata, West Bengal, India	108
17. Other Extramural Project	113
17.1. Hib Vaccine Probe Study -Part A	113
17.2. Rotavirous Genomics	114
17.3. A Multicentric Study On Surveillance And Molecular Characterization Of Human Caliciviruses(HuCVs) from Cases Of Acute Gastroenteritis	115
17.4. Genogrouping rotaviruses of human and animal origin in india	115
17.5. Porin of Shigella dysenteriae Mediated regulation of tool -like receptor and Costimulatory...	116
17.6. Study of the mechanism of transformation of vibro cholerae hemolysin...	116
17.7. Molecular vAspects of a 47-kDa N-acetyl-D- Glucosamine specific Vibrio cholerae Adhesin	117
17.8. Phase II and Phase III human trails with candidate oral vacine VA1.3-a multicentric study	117
17.9. Detection ,distribution and functional analysis of CTX prophages of toxigenic non -01, non-0139 vibrio......	118
17.10. Estimation of HIV load among different risk groups of West Bengal	118
18. Activities of the Training and Extension Division	120
18.1.Two training programmes for German Doctors on tropical and travel medicine jointly organized......	120
18.2 National Technology day calebration on may 11,2004	120
18.3. ph.D. orientation course from 14-18 June ,2004:	120
18.4 Training programme on Clinical managment and Diagnostic.../18.5. visit Mr Shogo Arai...	120
18.6. Four Extramural Training programmes on Immunization ......./18.7. WHO Biennium funded workshop on Data Based Generation on Disease Burden Estimation	121
18.8 Workshop of Immunology Study Group West Bengal/18.9. Training programme for the medical students of japan	122
18.10. Training promme of INFORMATICS/18.11 Organize the visit of students of Chaitanya PG College Andhra Pradesh	123
18.12. JICA-NICED Domestic Training programme on Molecular...../18.13. Training programme on Molecular Genetics of vibro......	123
18.14 3rd Country JICA-NICED Domestic Training programme on Molecular Epidemiology of .........	123
18.15 Training programme for WHO fellows for Research Methadology/18.16. Training programme for WHO fellows for .....	125
18.17 Training programme for Clinical Manajment of Acute Diarrhoea /18.18. Training programme for International WHO fellows for ....	126
18.19 Training programme for delhi...../ 18.20. Training programme for kolkata.....	126
18.21 ICMR-Lancet -IPHA (WB) workshop on medical paper writing/18.22. 3rd phase External Quality Assurance Scheme for HIV testing funded by NACO / 18.23 National Science Day celebration	126
18.24 All india meeting and workshop on phase III......./18.25. Training programme for the medical students of Japan/18.26. Organize the lecture of Guest Speakers	127
18.27 Summer Training programme ..../18.28. Organise the Institutionalseminars for the Scientsts and fellows	128
19. Scientific Conference/Seminar/Meeting Attended By The Scientists/Research Fellows/Staff	129
20. Training/Awards received by the Scientists/Fellows/Staff	170
21. Other activities of the Scientists	143
22. List of Publications	146
23. Committees of the Institute	151
24. List of the Staff	155